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Analysis of Eco RI Cleavage Patterns of Lambda DNA
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyze, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA
Analysis of Eco RI Cleavage Patterns of Lambda DNA
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyze, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA
Eigenschaften
- AP09-112/EDVO